Volume 8, Issue 2, June 2020, Page: 38-44
Manifestation of Functional Defects of Nervous System in Upf3 Mutants Drosophila melanogaster at Larval and Adult Stages
Sanusi Ahmed Jega, Faculty of Biomedical Science, Kampala International University, Ishaka-Bushenyi, Uganda; Department of Biochemistry, Faculty of Life Sciences, Kebbi State University of Science and Technology, Aliero, Nigeria
Ahmed Adebowole Adedeji, Faculty of Biomedical Science, Kampala International University, Ishaka-Bushenyi, Uganda; Foresight Institute of Research and Translation, Ibadan, Nigeria
Marta Vicente-Crespo, Institute of Biomedical Research, Kampala International University, Ishaka-Bushenyi, Uganda; African Population and Health Research Center, Nairobi, Kenya
Received: Mar. 30, 2020;       Accepted: Apr. 13, 2020;       Published: May 14, 2020
DOI: 10.11648/j.ab.20200802.12      View  297      Downloads  93
Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that cleans the system from possible harmful proteins and also regulates up to 10% of normal RNAs. The essential player proteins in the NMD (core NMD factors) are Upf1, Upf2, and Upf3. Mutation of any of these NMD factors cause ranges of effects in the development of various organisms. In humans, mutation of Upf3 was associated with neurodegenerative disorders, which include: attention deficit, schizophrenia autism, and intellectual disability. Using functional genetics approach and behavioral analysis methods we examined the loss of function effects of Upf3, in the nervous system function of a Drosophila melanogaster. We observed certain nervous system functional defects in homozygous Upf3 mutants. The embryos exhibited reduced and delayed hatching, the larvae manifested defects in motor function and the adults showed reduced climbing ability, defective short term memory, and learning, and notably, the adult life span was also reduced. This work has further revealed the prospect of Upf3 as a player gene for consideration in the management of neurodegenerative diseases. We explored this using Drosophila melanogaster as a model organism to mimic and study the neurodegenerative traits observed in the patients suffering from Upf3 mutation. Likewise, it suggests a further investigation into the mechanistic insight for the roles of Upf3 in both early and late CNS development.
Nonsense-mediated mRNA Decay, NMD Factors, Organismal Development, Neurodegenerative Behaviors, Drosophila melanogaster
To cite this article
Sanusi Ahmed Jega, Ahmed Adebowole Adedeji, Marta Vicente-Crespo, Manifestation of Functional Defects of Nervous System in Upf3 Mutants Drosophila melanogaster at Larval and Adult Stages, Advances in Biochemistry. Vol. 8, No. 2, 2020, pp. 38-44. doi: 10.11648/j.ab.20200802.12
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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