Volume 6, Issue 6, December 2018, Page: 47-52
Haptoglobin Polymorphism and Disturbance of Lipid Parameters in Victims of Strokes
Moustapha Djite, Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, University Cheikh Anta DIOP, Dakar, Senegal
Néné Oumou Kesso Barry, Laboratory of Biochemistry, University Hospital Fann, Dakar, Senegal
Jean Pascal Demba Diop, Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, University Cheikh Anta DIOP, Dakar, Senegal
Fatoumata Binta Bah, Laboratory of Biochemistry, University Hospital Fann, Dakar, Senegal
Pape Matar Kandji, Laboratory of Biochemistry, University Hospital Fann, Dakar, Senegal
El Hadji Malick Ndour, Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, University Cheikh Anta DIOP, Dakar, Senegal
Fatou Gueye-tall, Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, University Cheikh Anta DIOP, Dakar, Senegal
Dominique Doupa, Department of Medical Biochemistry, Saint-Louis University, Saint-Louis, Senegal
Rokhaya Ndiaye diallo, Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, University Cheikh Anta DIOP, Dakar, Senegal
Philomène Lopez Sall, Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, University Cheikh Anta DIOP, Dakar, Senegal
Aynina Cisse, Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, University Cheikh Anta DIOP, Dakar, Senegal
Pape Amadou Diop, Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, University Cheikh Anta DIOP, Dakar, Senegal
Papa Madièye Gueye, Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, University Cheikh Anta DIOP, Dakar, Senegal; Laboratory of Biochemistry, University Hospital Fann, Dakar, Senegal
Received: Feb. 17, 2019;       Accepted: Apr. 8, 2019;       Published: Apr. 29, 2019
DOI: 10.11648/j.ab.20180606.11      View  36      Downloads  21
Abstract
The objective of our study was to determine the frequency of the main genotypes of haptoglobin and their association with lipid parameters in subjects with stroke. This is a prospective, analytical and case-control study. 46 stroke patients and 46 matched controls were included by sex and age ± 2 years. Genotyping of Hp was performed using standard PCR with Proflex System PCR (Biosystems, Spain) and lipid parameters were assayed using enzymatic techniques with the Cobas c311 system (Roche, Germany). The mean age of our patients was 63 ± 14 years and the sex ratio was 0.70. Ischemic stroke was predominant with a frequency of 60.87% against 30.43% for hemorrhagic stroke. Allelic distribution in patients showed a high frequency of the Hp2 allele (51.09%) compared to control subjects (26.61%) (p <0,0001). The Hp2-2 genotype was more found in stroke subjects (36.96%) than in controls (17.39%) with a p = 0.002. A combination of the Hp2-2 genotype and the increase in mean LDL-c (p = 0.02), Triglycerides (p <0.005) and a decrease in mean HDL-c (p = 0.007) were found. Our study shows that the Hp2-2 genotype would be a predisposing factor to ischemic stroke. Its association with lipid parameters is found and could be considered as an additional risk factor.
Keywords
Stroke, Polymorphism of the Haptoglobin Gene, Lipid Balance
To cite this article
Moustapha Djite, Néné Oumou Kesso Barry, Jean Pascal Demba Diop, Fatoumata Binta Bah, Pape Matar Kandji, El Hadji Malick Ndour, Fatou Gueye-tall, Dominique Doupa, Rokhaya Ndiaye diallo, Philomène Lopez Sall, Aynina Cisse, Pape Amadou Diop, Papa Madièye Gueye, Haptoglobin Polymorphism and Disturbance of Lipid Parameters in Victims of Strokes, Advances in Biochemistry. Vol. 6, No. 6, 2018, pp. 47-52. doi: 10.11648/j.ab.20180606.11
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Faye E. H. O. «Aspects Tomodensitométriques des Accidents Vasculaires Cérébraux à l'hôpital régional de Kaolack» Thése d'exercice: Medecine, Dakar-Senegal: UCAD; 2016. 16
[2]
Adoukonou T. A., Vallat J. M., Joubert J., Macian F., Kabore R., Magy L., Houinato D., et Preux P-M. «Prise en charge des accidents vasculaires cérébraux en Afrique subsaharienne». Revue neurologique 2010; 166 (11): 882‑93.
[3]
Gaye A. «Accident Vasculaire Cérébral de la femme jeune à propos de 109 cas». Thése d'exercice: Medecine, Dakar-Senegal, UCAD; 2014.253.
[4]
Koch W., Latz W., Eichinger M., Roguin A., Levy A. P., Schömig A., Kastrati A. «Genotyping of the Common Haptoglobin Hp 1/2 Polymorphism Based on PCR». Clinical Chemistry 2002; 48 (9): 1377‑82.
[5]
Diouf Basse A. M., Ndao A. K., Ndiaye M., Touré K., Thiam A., Ndiaye M. M., Diop A. G., Ndiaye I. P.« Pronostic fonctionnel des accidents vasculaires cérébraux dans les pays en voie de développement: Sénégal». Annales de Réadaptation et de Médecine Physique 2006; 49 (3): 100‑104.
[6]
Mboup M. C., Sarr S. A., Dia K., Fall P. D. «Aspects étiologiques des accidents vasculaires cérébraux ischémiques au Sénégal». Pan African Medical Journal 2015; 22 (201).
[7]
Diarra E. A. A., Assouan A. E. K., Yao R. B., Kouame L. K., Kajo C., Tanoh C. «Épidémiologie des AVC en Côte d’Ivoire et perspectives». Revue Neurologique, Journées de Neurologie de langue française 2016;172: 164.
[8]
N’goran Y. N. K., Traore F., Tano M., Kramoh K, E., Kakou J. B. A., Konin C., Kakou M. G. «Aspects épidémiologiques des accidents vasculaires cérébraux (AVC) aux urgences de l’institut de cardiologie d’Abidjan (ICA)». The Pan African Medical Journal 2015;21.
[9]
Balogou A. A. K., Belo M. «Epidémiologie récente des accidents vasculaires cérébraux au Togo». Revue Neurologique, Journées de Neurologie de langue française 2015;171: 235‑36.
[10]
Bugnicourt J. M., Tchouata-Ngandjouc L., Beugre K., Chillon J-M., Godefroy O. «A comparison of stroke patients’ characteristics between a sub-Saharan Africa population (Abidjan) and a French stroke unit (Amiens): An observational study». Revue Neurologique 2013;169 (3): 258‑65.
[11]
Holme I., Aastveit A. H., Hammar N., Jungner I., Walldius G. «Haptoglobin and Risk of Myocardial Infarction, Stroke, and Congestive Heart Failure in 342,125 Men and Women in the Apolipoprotein MOrtality RISk Study (AMORIS)». Annals of Medicine 2009; 41 (7): 522‑32.
[12]
Standhave C., Svenson M., Kranup H., Christensen J. H. «Haptoglobine Genotype and Risk markers of CVD in patients with chronic kidney diseases». Hindavi Publishing Corporation, 2013; no 650847: 7pages.
[13]
Gueye PM, Bertrand F, Duportail G, Lessinger JM. Extracellular haemoglobin, oxidative stress and quality of red blood cells relative to perioperative blood salvage. Clin Chem Lab Med. 2010 May;48(5):677-83.,
[14]
Harris H., Robson E. B., Siniscalco M. «Genetics of the Plasma Protein Variants». In Ciba Foundation Symposium - Biochemistry of Human Genetics, édité par G. E. W. Wolstenholme O. B. E. M. R. C. P M. A., M. B. et Cecilia M. O’Connor B.Sc, 1959: 151‑77.
[15]
Moullec J., Fine J.-M., Linhard J. «Les groupes d’haptoglobine, moyen d’étude des populations humaines.» Bulletins et Mémoires de la Société d’anthropologie de Paris 1961; 2 (1): 109‑24.
[16]
Gordon D. J., Rifkinds B. M. « High-Density Lipoprotein--the Clinical Implications of Recent Studies ». The New England Journal of Medicine 1989; 321 (19): 1311‑16.
[17]
Soyama Y., Miura K., Morikawa Y., Nishijo M., Nakanishi Y., Naruse Y., Kagamimori S., Nakagawa H. «High-Density Lipoprotein Cholesterol and Risk of Stroke in Japanese Men and Women». Stroke 2003; 34 (4): 863‑68.
[18]
McGrowder D., Riley C., Morrison E. Y. St A., Gordon L. «The Role of High-Density Lipoproteins in Reducing the Risk of Vascular Diseases, Neurogenerative Disorders, and Cancer». Cholesterol 2010: 1-9.
[19]
Rohatgi A., Khera A., Berry J. D., Givens E. G., Ayers C. R., Wedin K. E., Ian J. Neeland I. J., Yuhunna I. S., Rader D. R., De emos J. A., Shaul P. W. «HDL Cholesterol Efflux Capacity and Incident Cardiovascular Events». New England Journal of Medicine 2014; 371 (25): 2383‑93.
[20]
Cisse O., Lemine D. S. M., Ba F., Ba E. H. M., Diop M. S., Diagne N. S., Sow A. D., Basse A. M., Touré K., Ndiaye M., Diop G. A., Ndiaye M. M. «Le profil lipidique et glucidique des accidents vasculaires cérébraux ischémiques à Dakar». The Pan African Medical Journal 2016;25.
[21]
Gotto Jr A. M., Brinton E. A. «Assessing low levels of high-density lipoprotein cholesterol as a risk factor in coronary heart disease: A working group report and update». Journal of the American College of Cardiology 2004; 43 (5): 717‑24.
[22]
Hovingh G. K., Hutten B. A., Holleboom A. G., Petersen W., Rol P., Stalenhoef A., Zwinderman A. H., De Groot E., Kastelein J. J. P., Kuivenhoven J. A.«Compromised LCAT Function Is Associated With Increased Atherosclerosis». Circulation 2005; 112 (6): 879‑84.
[23]
Lindenstrøm E., Boysen G., Nyboe J. «Influence of Total Cholesterol, High Density Lipoprotein Cholesterol, and Triglycerides on Risk of Cerebrovascular Disease: The Copenhagen City Heart Study». BMJ (Clinical Research Ed.) 1994; 309 (6946): 11‑15.
[24]
Sow A. D., Basse A. M., Sarr M. M., Cisse O., Moustapha N., Diop A. G., & Mouhamadou M. N. Variations métaboliques, place de l’haptoglobine chez des épileptiques, versus témoins, suivis au CHUN de Fann; Dakar, Senegal. Revue Neurologique 2017; 173 (2): 46.
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