,
Adon Mousan Arsène,
Apoline Jeannette Aka Adouko,
Phillipe Alain Dit Bidié,
Okpèkon Aboua Timothée
Bacground: Nauclea latifolia is a medicinal plant used in Côte d'Ivoire as a remedy for tooth decay, dental pain and mouth sores. Its consumption can be toxic. Objective: The aim of this study was to assess the renal, hepatic and myocardial effects, in order to prevent toxicity. Materials and methods: Phytochemistry was carried out by phytochemical screening and thin-layer chromatography. Acute and sub-acute toxicity tests were carried out in accordance with OECD protocols 423 and 407 respectively. Blood samples were taken on days 14 and 28 for analysis of biochemical parameters. Urea was determined using the enzymatic method described by Tietz. Creatinine was determined using the method of Jaffé. Total plasma protein concentration called the “biuret method” developed by Kronh. Alanine aminotransferase activity was determined according to the method recommended by the International Federation of Clinical Chemistry (IFCC). Aspartate aminotransferase (ASAT) activity was determined according to the method of ECCLS. Results: Phytochemistry showed the qualitative and expressive presence of polyphenols, flavonoids, tannins, phenolic acids and saponosides. For acute toxicity, somnolence was reported. No mortality or morbidity was observed. The LD50 was greater than 5000 mg/kg. Blood results showed no temporal disturbance in the functioning of biochemical markers of kidney, liver and heart, but some marginal variations were observed. Conclusion: The non-toxic effect revealed is proportional to the dose and active substances of the aqueous extract of Nauclea latifolia stem bark. This experiment would enable us to formulate improved traditional medicines to help treat oral cavity disorders.
| Published in | Advances in Biochemistry (Volume 13, Issue 3) |
| DOI | 10.11648/j.ab.20251303.12 |
| Page(s) | 79-86 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2025. Published by Science Publishing Group |
Nauclea Latifolia Stem Bark, Toxicity Studies, Phytochemicals, Biochemical Parameters, Biochemical Markers
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APA Style
Angeline, A. K. M., Ohoueu, H. S. S., Arsène, A. M., Adouko, A. J. A., Bidié, P. A. D., et al. (2025). Photochemistry and Toxicity Studies of Aqueous Nauclea Latifolia Stem Bark Extract: Effect on Serum Biochemical Markers in Wistar Rats. Advances in Biochemistry, 13(3), 79-86. https://doi.org/10.11648/j.ab.20251303.12
ACS Style
Angeline, A. K. M.; Ohoueu, H. S. S.; Arsène, A. M.; Adouko, A. J. A.; Bidié, P. A. D., et al. Photochemistry and Toxicity Studies of Aqueous Nauclea Latifolia Stem Bark Extract: Effect on Serum Biochemical Markers in Wistar Rats. Adv. Biochem. 2025, 13(3), 79-86. doi: 10.11648/j.ab.20251303.12
AMA Style
Angeline AKM, Ohoueu HSS, Arsène AM, Adouko AJA, Bidié PAD, et al. Photochemistry and Toxicity Studies of Aqueous Nauclea Latifolia Stem Bark Extract: Effect on Serum Biochemical Markers in Wistar Rats. Adv Biochem. 2025;13(3):79-86. doi: 10.11648/j.ab.20251303.12
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Download@article{10.11648/j.ab.20251303.12,
author = {Angbo Kousso Marie Angeline and Honoré St Seka Ohoueu and Adon Mousan Arsène and Apoline Jeannette Aka Adouko and Phillipe Alain Dit Bidié and Okpèkon Aboua Timothée},
title = {Photochemistry and Toxicity Studies of Aqueous Nauclea Latifolia Stem Bark Extract: Effect on Serum Biochemical Markers in Wistar Rats
},
journal = {Advances in Biochemistry},
volume = {13},
number = {3},
pages = {79-86},
doi = {10.11648/j.ab.20251303.12},
url = {https://doi.org/10.11648/j.ab.20251303.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20251303.12},
abstract = {Bacground: Nauclea latifolia is a medicinal plant used in Côte d'Ivoire as a remedy for tooth decay, dental pain and mouth sores. Its consumption can be toxic. Objective: The aim of this study was to assess the renal, hepatic and myocardial effects, in order to prevent toxicity. Materials and methods: Phytochemistry was carried out by phytochemical screening and thin-layer chromatography. Acute and sub-acute toxicity tests were carried out in accordance with OECD protocols 423 and 407 respectively. Blood samples were taken on days 14 and 28 for analysis of biochemical parameters. Urea was determined using the enzymatic method described by Tietz. Creatinine was determined using the method of Jaffé. Total plasma protein concentration called the “biuret method” developed by Kronh. Alanine aminotransferase activity was determined according to the method recommended by the International Federation of Clinical Chemistry (IFCC). Aspartate aminotransferase (ASAT) activity was determined according to the method of ECCLS. Results: Phytochemistry showed the qualitative and expressive presence of polyphenols, flavonoids, tannins, phenolic acids and saponosides. For acute toxicity, somnolence was reported. No mortality or morbidity was observed. The LD50 was greater than 5000 mg/kg. Blood results showed no temporal disturbance in the functioning of biochemical markers of kidney, liver and heart, but some marginal variations were observed. Conclusion: The non-toxic effect revealed is proportional to the dose and active substances of the aqueous extract of Nauclea latifolia stem bark. This experiment would enable us to formulate improved traditional medicines to help treat oral cavity disorders.},
year = {2025}
}
TY - JOUR T1 - Photochemistry and Toxicity Studies of Aqueous Nauclea Latifolia Stem Bark Extract: Effect on Serum Biochemical Markers in Wistar Rats AU - Angbo Kousso Marie Angeline AU - Honoré St Seka Ohoueu AU - Adon Mousan Arsène AU - Apoline Jeannette Aka Adouko AU - Phillipe Alain Dit Bidié AU - Okpèkon Aboua Timothée Y1 - 2025/07/18 PY - 2025 N1 - https://doi.org/10.11648/j.ab.20251303.12 DO - 10.11648/j.ab.20251303.12 T2 - Advances in Biochemistry JF - Advances in Biochemistry JO - Advances in Biochemistry SP - 79 EP - 86 PB - Science Publishing Group SN - 2329-0862 UR - https://doi.org/10.11648/j.ab.20251303.12 AB - Bacground: Nauclea latifolia is a medicinal plant used in Côte d'Ivoire as a remedy for tooth decay, dental pain and mouth sores. Its consumption can be toxic. Objective: The aim of this study was to assess the renal, hepatic and myocardial effects, in order to prevent toxicity. Materials and methods: Phytochemistry was carried out by phytochemical screening and thin-layer chromatography. Acute and sub-acute toxicity tests were carried out in accordance with OECD protocols 423 and 407 respectively. Blood samples were taken on days 14 and 28 for analysis of biochemical parameters. Urea was determined using the enzymatic method described by Tietz. Creatinine was determined using the method of Jaffé. Total plasma protein concentration called the “biuret method” developed by Kronh. Alanine aminotransferase activity was determined according to the method recommended by the International Federation of Clinical Chemistry (IFCC). Aspartate aminotransferase (ASAT) activity was determined according to the method of ECCLS. Results: Phytochemistry showed the qualitative and expressive presence of polyphenols, flavonoids, tannins, phenolic acids and saponosides. For acute toxicity, somnolence was reported. No mortality or morbidity was observed. The LD50 was greater than 5000 mg/kg. Blood results showed no temporal disturbance in the functioning of biochemical markers of kidney, liver and heart, but some marginal variations were observed. Conclusion: The non-toxic effect revealed is proportional to the dose and active substances of the aqueous extract of Nauclea latifolia stem bark. This experiment would enable us to formulate improved traditional medicines to help treat oral cavity disorders. VL - 13 IS - 3 ER -
Department of Bioclinical and Fundamental Sciences, Training and Research Unit of Medical Sciences, Medical Biochemistry Laboratory, Félix Houphouët Boigny University, Abidjan, Côte d’Ivoire
Doctoral School of Biology, Environment and Health, Félix Houphouët Boigny University (UFHB), Abidjan, Ivory Coast
Unit of Cellular Biology Pasteur Institute of Adiopodoumé Ivory Coast, Abidjan, Côte d'Ivoire
Department of Biology, Periodontology and Oral Medicine, UFR Odontostomatology Félix Houphouet Boigny University (UFHB), Abidjan, Ivory Coast
Pharmacology of Natural Substances UFR Biosciences, Félix Houphouët Boigny University (UFHB), Abidjan, Ivory Coast
Pharmacology, Pharmacognosy of Constitution and Reaction of Matter, Laboratory of Biology and Health, Félix Houphouët Boigny University (UFHB), Abidjan, Ivory Coast
